1S13
Human Heme Oxygenase Oxidatition of alpha- and gamma-meso-Phenylhemes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 119 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-11-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.070, 75.750, 107.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.290 |
| Rwork | 0.210 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.066 | 0.038 |
| Number of reflections | 20818 | |
| <I/σ(I)> | 21.1 | 2.12 |
| Completeness [%] | 99.9 | 95 |
| Redundancy | 9.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 301 | ammonium sulphate, HEPES, hexanediol, jeffamine ED600, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
