1S0Q
Native Bovine Pancreatic Trypsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.383, 58.710, 66.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.020 |
| R-factor | 0.1122 |
| Rwork | 0.111 |
| R-free | 0.14160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1j8a |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.060 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Number of reflections | 101386 | |
| Completeness [%] | 93.5 | 77.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | PEG 6000, Ammonium Sulphate, Calcium Chloride, TRIS-HCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
