1S0H
Structure determination of haemoglobin from Donkey(equus asinus) at 3.0 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.664, 63.084, 54.042 |
| Unit cell angles | 90.00, 111.75, 90.00 |
Refinement procedure
| Resolution | 21.180 - 3.000 |
| R-factor | 0.1857 |
| Rwork | 0.181 |
| R-free | 0.28524 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Horse Methaemoglobin(PDB ID:2MHB) |
| RMSD bond length | 0.041 |
| RMSD bond angle | 3.149 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.079 |
| High resolution limit [Å] | 3.002 | 3.002 |
| Rmerge | 0.161 | 0.430 |
| Number of reflections | 5387 | |
| <I/σ(I)> | 4.3 | |
| Completeness [%] | 79.0 | 34.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 2.8M Na/K phosphate buffer, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
