1S07
Crystal Structure of the R253A Mutant of 7,8-Diaminopelargonic Acid Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.85 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.080, 56.527, 120.993 |
| Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 * |
| R-factor | 0.18968 |
| Rwork | 0.188 |
| R-free | 0.22500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WT dimer |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.480 |
| High resolution limit [Å] | 2.400 * | 2.420 |
| Rmerge | 0.077 * | 0.248 * |
| Total number of observations | 86216 * | |
| Number of reflections | 26936 | |
| <I/σ(I)> | 14.8 | 24.8 |
| Completeness [%] | 90.6 | 55.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | PEG4000, MPD, HEPES, isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 26-28 (%) | |
| 2 | 1 | reservoir | MPD | 9-12 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | drop | protein | 10 (mg/ml) |
