1S02
EFFECTS OF ENGINEERED SALT BRIDGES ON THE STABILITY OF SUBTILISIN BPN'
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.300, 72.900, 75.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.900 |
| R-factor | 0.169 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.031 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.085 * |
| Total number of observations | 59579 * |
| Number of reflections | 9999 * |
| Completeness [%] | 56.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PMSF-inhibited enzyme | 7 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 35-40 (%sat) | |
| 4 | 1 | reservoir | PEG8000 | 0.5 (%) | |
| 5 | 1 | reservoir | beta-octylglucoside | 1 (%) | |
| 6 | 1 | reservoir | MPD | 2 (%) | |
| 7 | 1 | reservoir | MES | 50 (mM) |
