1RZU
Crystal structure of the glycogen synthase from A. tumefaciens in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2003-08-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9393 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.357, 88.115, 84.463 |
| Unit cell angles | 90.00, 98.19, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.18434 |
| Rwork | 0.180 |
| R-free | 0.22328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Non complexed form of glycogen synthase (Agrobacterium tumefaciens) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.804 |
| Data reduction software | MOSFLM (V. 6.2.0) |
| Data scaling software | CCP4 ((SCALA) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.337 |
| Number of reflections | 40385 | |
| <I/σ(I)> | 6.9 | 2.9 |
| Completeness [%] | 90.7 | 85.5 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, isopropanol, HEPES, ADP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
