1RZN

Crystal Structure of Penicillin-binding protein-related factor A from Bacillus Subtilis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	1.54000
Spacegroup name	P 65
Unit cell lengths	74.303, 74.303, 155.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.330 - 2.302
R-factor	0.18897
Rwork	0.186
R-free	0.24507
Structure solution method	SIRAS
RMSD bond length	0.028
RMSD bond angle	2.186
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.302
Rmerge	0.086
Number of reflections	17965
<I/σ(I)>	21.4
Completeness [%]	98.1
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 8000, Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	PEG 8000, Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K