1RZG
Crystal structure of Human anti-HIV-1 GP120 reactive antibody 412d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.088, 89.313, 85.863 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2011 |
| Rwork | 0.201 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.395 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.069 * | |
| Total number of observations | 209967 * | |
| Number of reflections | 66722 * | |
| Completeness [%] | 92.5 * | 58.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | 18% PEG 4000, 0.1M NaCl, 0.02M Calcium chloride, 0.3M sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 2 | 1 | drop | 0.35 (M) | ||
| 3 | 1 | drop | 0.02 (%(v/v)) | pH7.0 | |
| 4 | 1 | drop | protein | 4-8 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 18 (%) | |
| 6 | 1 | reservoir | 0.1 (M) | ||
| 7 | 1 | reservoir | 0.02 (M) | ||
| 8 | 1 | reservoir | sodium acetate | 0.3 (M) | pH6.0 |
