1RZ7
CRYSTAL STRUCTURE OF HUMAN ANTI-HIV-1 GP120-REACTIVE ANTIBODY 48D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.485, 72.951, 101.921 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.2011 |
| Rwork | 0.201 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.386 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 * | |
| Total number of observations | 230911 * | |
| Number of reflections | 32647 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 99.8 * | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | 8.3% PEG4000, 8.3% isopropanol, 0.33M NaCl, 0.04M sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 2.5 (mM) | |
| 2 | 1 | drop | 0.35 (M) | ||
| 3 | 1 | drop | 0.02 (%(v/v)) | pH7.0 | |
| 4 | 1 | drop | protein | 4-8 (mg/ml) | |
| 5 | 1 | reservoir | PEG4000 | 8.3 (%) | |
| 6 | 1 | reservoir | isopropyl alcohol | 8.3 (%) | |
| 7 | 1 | reservoir | 0.33 (M) | ||
| 8 | 1 | reservoir | sodium citrate | 0.04 (M) | pH5.6 |
