1RZ3
Structure of a Possible Uridine Kinase from Bacillus stearothermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97896, 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.010, 45.185, 107.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.450 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.23800 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.450 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 17505 | |
| Completeness [%] | 83.7 | 93.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 2-propanol, MES pH6.0, calcium acetate, ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
