1RYX

Crystal structure of hen serum transferrin in apo- form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-02-15
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	90.295, 90.295, 177.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.690 - 3.500
R-factor	0.283
Rwork	0.283
R-free	0.34100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aiv
RMSD bond length	0.011
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.480
High resolution limit [Å]	3.400	3.400
Rmerge	0.102	0.453
Number of reflections	10753
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	277	18-23% PEG 6000 in 0.02M sodium acetate buffer, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 277K