1RYP
CRYSTAL STRUCTURE OF THE 20S PROTEASOME FROM YEAST AT 2.4 ANGSTROMS RESOLUTION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.490, 300.700, 144.420 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.286 |
| Rwork | 0.286 |
| R-free | 0.33000 |
| Starting model (for MR) | 1pma |
| RMSD bond length | 0.011 |
| RMSD bond angle | 23.100 * |
| Data reduction software | MOSFLM (V. 5.3) |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.900 | 1.970 |
| Rmerge | 0.081 | 0.487 |
| Number of reflections | 778118 | |
| <I/σ(I)> | 2 | 2 |
| Completeness [%] | 93.6 | 93.6 |
| Redundancy | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.9 | 24 * | pH 6.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 27 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 6.7 (mM) | |
| 3 | 1 | drop | EDTA | 0.7 (mM) | |
| 4 | 1 | drop | magnesium acetate | 13 (mM) | |
| 5 | 1 | drop | morpholino ethane sulphonic acid | 0.03 (M) | |
| 6 | 1 | drop | 2,4-methylpentanediol | 4 (%) | |
| 7 | 1 | reservoir | magnesium acetate | 40 (mM) | |
| 8 | 1 | reservoir | morpholino ethane sulphonic acid | 0.1 (M) | |
| 9 | 1 | reservoir | 2,4-methylpentanediol | 12 (%) |
