1RYM
Structure of the Group II Intron Splicing Factor CRS2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-06 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 53.100, 79.600, 112.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 * - 1.800 |
R-factor | 0.195 |
Rwork | 0.190 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CRS2 residues through K178 (PTH numbering) |
RMSD bond length | 0.025 * |
RMSD bond angle | 2.800 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | EPMR |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 * | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.069 * | 0.257 * |
Number of reflections | 22475 | |
<I/σ(I)> | 5.7 | 2.5 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.9 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 10% PEG 3400, sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG3400 | 10 (%) | |
3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.5 |