1RYF

Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	51.640, 78.690, 96.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.920 - 1.750
R-factor	0.1899
Rwork	0.188
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mh1
RMSD bond length	0.021
RMSD bond angle	1.870 *
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.920 *
High resolution limit [Å]	1.750	1.750 *
Rmerge	0.054 *	0.409 *
Total number of observations	130220 *
Number of reflections	38560 *
Completeness [%]	95.1 *	88.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH7.5
2	1	drop		2 (mM)
3	1	drop	Rac1b	0.5 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	drop	GDP	100000 (nM)
6	1	reservoir	HEPES	100 (mM)	pH7.5
7	1	reservoir	PEG3350	18-30 (%)
8	1	reservoir	isopropanol	2-6 (%)