1RYF
Alternative Splicing of Rac1 Generates Rac1b, a Self-activating GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.640, 78.690, 96.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.920 - 1.750 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mh1 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.870 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.920 * | |
| High resolution limit [Å] | 1.750 | 1.750 * |
| Rmerge | 0.054 * | 0.409 * |
| Total number of observations | 130220 * | |
| Number of reflections | 38560 * | |
| Completeness [%] | 95.1 * | 88.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH7.5 |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | Rac1b | 0.5 (mM) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | GDP | 100000 (nM) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | reservoir | PEG3350 | 18-30 (%) | |
| 8 | 1 | reservoir | isopropanol | 2-6 (%) |
