1RYE
Crystal Structure of the Shifted Form of the Glucose-Fructose Oxidoreductase from Zymomonas mobilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-22 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.868, 152.980, 101.992 |
Unit cell angles | 90.00, 103.66, 90.00 |
Refinement procedure
Resolution | 49.560 - 2.300 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ofg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Data reduction software | d*TREK |
Data scaling software | HKL-2000 |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.060 | 0.239 |
Number of reflections | 54903 | |
<I/σ(I)> | 8.1 | 3.4 |
Completeness [%] | 83.4 | 49.2 |
Redundancy | 3.5 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 4000, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |