1RYD

Crystal Structure of Glucose-Fructose Oxidoreductase from Zymomonas mobilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-22
Detector	SBC-2
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	84.525, 91.382, 115.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.680 - 2.200
R-factor	0.209
Rwork	0.204
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ofg
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.122	0.560
Number of reflections	45279
<I/σ(I)>	7.2	2.7
Completeness [%]	98.6	94.1
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	PEG 4000, ammonium acetate, sodium citrate, glucose, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K