1RYB

Crystal Structure of the Chloroplast Group II Intron Splicing Factor CRS2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-12-02
Detector	MARRESEARCH
Wavelength(s)	0.773
Spacegroup name	P 21 21 21
Unit cell lengths	36.375, 62.644, 78.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.100 * - 1.700
R-factor	0.176
Rwork	0.173
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pth
RMSD bond length	0.021
RMSD bond angle	2.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.100 *	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.052 *	0.194 *
Number of reflections	20160 *
<I/σ(I)>	8.1	3.9
Completeness [%]	99.6	99.6
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	298	PEG 4000, CAPS, sodium chloride, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG3400	24 (%)
3	1	reservoir		100 (mM)
4	1	reservoir	CAPS	100 (mM)	pH10.5