1RYB
Crystal Structure of the Chloroplast Group II Intron Splicing Factor CRS2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.773 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.375, 62.644, 78.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.100 * - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pth |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.100 * | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 * | 0.194 * |
| Number of reflections | 20160 * | |
| <I/σ(I)> | 8.1 | 3.9 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | PEG 4000, CAPS, sodium chloride, pH 10.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3400 | 24 (%) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | CAPS | 100 (mM) | pH10.5 |
