1RY6
Crystal Structure of Internal Kinesin Motor Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1272 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.588, 105.588, 84.771 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.920 - 1.600 |
| R-factor | 0.20676 |
| Rwork | 0.205 |
| R-free | 0.23128 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | KAR3 PDB entry 1F9T |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.855 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.690 |
| High resolution limit [Å] | 1.500 | 1.620 |
| Number of reflections | 77888 | |
| <I/σ(I)> | 15.52 | 1.75 |
| Completeness [%] | 90.3 | 84.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | ammonium sulfate, sodium nitrate, sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
