1RY2
Crystal structure of yeast acetyl-coenzyme A synthetase in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 3 |
| Unit cell lengths | 107.130, 107.130, 54.790 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 * - 2.300 |
| R-factor | 0.1991 |
| Rwork | 0.199 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pg4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.066 * | 0.240 * |
| Total number of observations | 85039 * | |
| Number of reflections | 27899 | |
| Completeness [%] | 89.0 * | 79 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 4 * | succinic acid, tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 271K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | succinic acid | 0.7 (M) | |
| 2 | 1 | reservoir | Tris | 0.1 (M) | pH7.5 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | ATP | 5 (mM) |
