1RY0
Structure of prostaglandin F synthase with prostaglandin D2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2003-08-12 |
| Wavelength(s) | 1.02 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.970, 49.180, 83.520 |
| Unit cell angles | 73.80, 85.90, 69.80 |
Refinement procedure
| Resolution | 10.000 - 1.690 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3-alpha-HSD (PDB code: 1AFS) |
| RMSD bond length | 0.060 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 74430 | |
| Completeness [%] | 95.8 | 75.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | PEG 8000, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
