1RXZ

C-terminal region of A. fulgidus FEN-1 complexed with A. fulgidus PCNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-15
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1159
Spacegroup name	H 3
Unit cell lengths	86.530, 86.530, 96.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.000
R-factor	0.189
Rwork	0.180
R-free	0.26000 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rwz
RMSD bond length	0.006
RMSD bond angle	1.290
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.100
High resolution limit [Å]	2.000	2.000
Number of reflections	36466
<I/σ(I)>		2.2
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	21 *	Na/K phosphate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	Tris	50 (mM)	pH8.0
3	1	drop		250 (mM)
4	1	reservoir	sodium/pootassium phosphate	1.4-1.6 (M)	pH9.0