1RXS
E. coli uridine phosphorylase: 2'-deoxyuridine phosphate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 115 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-08-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 160.958, 98.212, 242.729 |
| Unit cell angles | 90.00, 109.09, 90.00 |
Refinement procedure
| Resolution | 26.750 - 2.800 |
| R-factor | 0.17794 |
| Rwork | 0.176 |
| R-free | 0.21814 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DETWINNED HEXAMERIC NATIVE UP |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.490 | 2.940 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.123 | 0.413 |
| Number of reflections | 173525 | |
| <I/σ(I)> | 5.4 | 1.8 |
| Completeness [%] | 97.8 | 95.3 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD UNDER OIL | 7.5 | 289 | TRIS HCL, PEG4000, POTASSIUM ACETATE, 2'-DEOXYURIDINE, SODIUM ORTHOVANADATE, pH 7.50, BATCH METHOD UNDER OIL, temperature 289K |
