1RXM
C-terminal region of FEN-1 bound to A. fulgidus PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-08-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.855 |
Spacegroup name | H 3 2 |
Unit cell lengths | 101.127, 101.127, 203.148 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.211 |
Rwork | 0.205 |
R-free | 0.26100 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rwz |
RMSD bond length | 0.006 |
RMSD bond angle | 1.290 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11174 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 21 * | Na/K phosphate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
3 | 1 | drop | 250 (mM) | ||
4 | 1 | reservoir | sodium/pootassium phosphate | 1.4-1.6 (M) | pH9.0 |