1RXK
crystal structure of streptavidin mutant (M3) a combination of M1+M2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-12-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.003 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.335, 81.579, 90.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.700 |
| R-factor | 0.20153 |
| Rwork | 0.200 |
| R-free | 0.23905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.647 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.800 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 31858 | |
| Completeness [%] | 97.0 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 293 | 1.5M AS, 0.1M Tris-HCl, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
