1RXJ
Crystal structure of streptavidin mutant (M2) where the L3,4 loop was replace by that of avidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2002-12-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.003 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.355, 85.080, 57.694 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 56.800 - 1.140 |
R-factor | 0.18022 |
Rwork | 0.179 |
R-free | 0.20029 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.247 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 56.800 |
High resolution limit [Å] | 1.140 |
Number of reflections | 148143 |
<I/σ(I)> | 12.4 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 4000, 0.1M NaAc, 0.1M NaCac, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |