1RXH
Crystal structure of streptavidin mutant L124R (M1) complexed with biotinyl p-nitroanilide (BNI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-20 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 56.385, 56.385, 238.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.900 |
| R-factor | 0.218 |
| Rwork | 0.212 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 5466 | |
| Completeness [%] | 97.9 | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
