1RXH
Crystal structure of streptavidin mutant L124R (M1) complexed with biotinyl p-nitroanilide (BNI)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2002-10-20 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 56.385, 56.385, 238.571 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.218 |
Rwork | 0.212 |
R-free | 0.30900 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 5466 | |
Completeness [%] | 97.9 | 98.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |