1RWZ
Crystal Structure of Proliferating Cell Nuclear Antigen (PCNA) from A. fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.2398 |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 244.355, 244.355, 244.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Model built from 2.1 angstrom resolution data phased by a single anomalous derivative (ethyl mercury phosphate) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.263 |
| Number of reflections | 56483 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 97.3 | 96.6 |
| Redundancy | 93.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 21 * | Na/K phosphate, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8.0 |
| 3 | 1 | drop | 250 (mM) | ||
| 4 | 1 | reservoir | sodium/pootassium phosphate | 1.4-1.6 (M) | pH9.0 |
