1RWC
Crystal structure of Arthrobacter aurescens chondroitin AC lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.679, 86.437, 81.289 |
| Unit cell angles | 90.00, 106.88, 90.00 |
Refinement procedure
| Resolution | 29.360 - 1.900 |
| R-factor | 0.19135 |
| Rwork | 0.190 |
| R-free | 0.25178 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rw9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.677 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 60174 | |
| <I/σ(I)> | 7.3 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | PEG 8000, ammonium acetate, glycerol, phosphate buffer, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
