1RWA
Crystal structure of Arthrobacter aurescens chondroitin AC lyase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.005133, 1.009078, 0.997068 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.356, 85.256, 82.162 |
| Unit cell angles | 90.00, 105.78, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.300 |
| R-factor | 0.134 |
| Rwork | 0.134 |
| R-free | 0.15600 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.08) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 183424 | |
| <I/σ(I)> | 14.7 | 6.8 |
| Completeness [%] | 98.8 | 89 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 293 | PEG 8000, ammonium acetate, glycerol, phosphate buffer, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 6.40 |
