1RW4
Nitrogenase Fe protein l127 deletion variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Wavelength(s) | 2.5 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.780, 133.090, 61.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.27200 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g20 |
RMSD bond length | 0.010 * |
RMSD bond angle | 2.700 * |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.500 | |
Rmerge | 0.059 * | 0.135 * |
Total number of observations | 122724 * | |
Number of reflections | 10015 | |
Completeness [%] | 99.4 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 8.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 70 (mg/ml) | |
2 | 1 | 1 | PEG4000 | 24 (%) | |
3 | 1 | 1 | 0.16 (M) | pH8.5 | |
4 | 1 | 1 | trishydrochloric acid | ||
5 | 1 | 1 | glycerol | 20 (%) | |
6 | 1 | 1 | sodium dithionite | 1 (mM) |