1RW1
YFFB (PA3664) PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-30 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.450, 43.250, 29.060 |
Unit cell angles | 90.00, 93.50, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.020 |
R-factor | 0.129 |
Rwork | 0.129 |
R-free | 0.13900 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.330 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.040 |
High resolution limit [Å] | 1.020 | 1.020 |
Rmerge | 0.051 | 0.419 |
Number of reflections | 98866 | |
<I/σ(I)> | 22 | 3.6 |
Completeness [%] | 91.2 | 92.3 |
Redundancy | 3.9 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 10 | 295 | 0.1M CHES, 26% PEG 3350, 5% isopropanol, pH 10, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 10.00 |