1RVZ

1934 H1 Hemagglutinin in complex with LSTC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-20
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	227.583, 131.522, 175.017
Unit cell angles	90.00, 110.07, 90.00

Refinement procedure

Resolution	20.000 - 2.250
R-factor	0.225
Rwork	0.225
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.082	0.401
Number of reflections	206016
<I/σ(I)>	14.6	2.9
Completeness [%]	89.9	52.2
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	PEG3350, TrisHCl, KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K