1RVV

SYNTHASE/RIBOFLAVIN SYNTHASE COMPLEX OF BACILLUS SUBTILIS

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	235.900, 192.600, 168.600
Unit cell angles	90.00, 134.90, 90.00

Refinement procedure

Resolution	10.000 * - 2.400
R-factor	0.237
Rwork	0.237
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400
Rmerge	0.126 *
Total number of observations	18350500 *
Number of reflections	122010 *
Completeness [%]	51.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-8 (mg/ml)
2	1	drop	sodium/potassium phosphate	0.8 (M)
3	1	drop	5-nitro-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	1 (mM)
4	1	reservoir	sodium/potassium phosphate	1.55 (M)