1RVU

E75Q MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-02-27
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	114.650, 114.650, 156.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.330 - 2.500
R-factor	0.223
Rwork	0.222
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cj0
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DPS
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.300	2.640
High resolution limit [Å]	2.500	2.500
Number of reflections	36699
<I/σ(I)>	6.4	1.4
Completeness [%]	99.6	99.3
Redundancy	5.3	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	298	PEG 4000, KCl, KMES, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K