1RVT

1930 H1 Hemagglutinin in complex with LSTC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-01
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	204.296, 83.300, 177.380
Unit cell angles	90.00, 106.15, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.217
Rwork	0.217
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.087	0.409
Number of reflections	97348
<I/σ(I)>	14.5	2.2
Completeness [%]	98.2	93.6
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291	PEG 5K MME, TrisHCl, Sodium citrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K