1RVJ

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG H177 REPLACED WITH HIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.95
Spacegroup name	P 31 2 1
Unit cell lengths	138.242, 138.242, 184.396
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.000 - 2.750
R-factor	0.222
Rwork	0.218
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qov
RMSD bond length	0.011
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.900
High resolution limit [Å]	2.750	2.750
Number of reflections	53191
<I/σ(I)>	7.4	2
Completeness [%]	99.6	99.4
Redundancy	6.8	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	potassium phosphate, sodium chloride, haptanetriol, dioxane, haxanetriol, LDAO, pH 8.50, VAPOR DIFFUSION, SITTING DROP, temperature 292K