1RVF
FAB COMPLEXED WITH INTACT HUMAN RHINOVIRUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11-03 |
| Detector | FUJI |
| Spacegroup name | R 3 |
| Unit cell lengths | 372.000, 372.000, 372.000 |
| Unit cell angles | 108.40, 108.40, 108.40 |
Refinement procedure
| Resolution | 10.000 - 4.000 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SEE REFERENCE 1 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | ENVELOPE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 4.180 |
| High resolution limit [Å] | 4.000 * | 4.000 |
| Rmerge | 0.166 | |
| Number of reflections | 259123 | |
| Completeness [%] | 64.5 | 46.9 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 2 | 1 | drop | 250-300 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | PEG8000 | 0.75 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 0.75 (%) |
