1RVB

MG2+ BINDING TO THE ACTIVE SITE OF ECO RV ENDONUCLEASE: A CRYSTALLOGRAPHIC STUDY OF COMPLEXES WITH SUBSTRATE AND PRODUCT DNA AT 2 ANGSTROMS RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 1
Unit cell lengths	49.160, 50.420, 64.060
Unit cell angles	96.35, 108.85, 108.45

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.165
Rwork	0.165
RMSD bond length	0.010
RMSD bond angle	0.770 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.052 *
Total number of observations	40175 *
Number of reflections	26271 *
Completeness [%]	83.7 *	65 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	EcoRV	15 (mg/ml)
2	1	1	phosphate	10 (mM)
3	1	1		250 (mM)
4	1	1	EDTA	1 (mM)
5	1	1	DTT	0.1 (mM)
6	1	1	oligodeoxynucleotide	3.0 (mg/ml)
7	1	1	cacodylate	10 (mM)
8	1	1	PEG4000	0.8-1.4 (%)	final concentrayions