1RV9

Crystal Structure of Neisseria meningitidis protein NMB0706, Pfam DUF152

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-10
Detector	BRANDEIS - B4
Wavelength(s)	0.9792, 0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	78.850, 41.340, 72.140
Unit cell angles	90.00, 99.34, 90.00

Refinement procedure

Resolution	50.000 - 1.530
Rwork	0.197
R-free	0.20950
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.589
Data reduction software	MARMAD
Data scaling software	SCALEPACK
Phasing software	SOLVE (& SHARP)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.530	1.530
Rmerge	0.027	0.191
Number of reflections	33998
<I/σ(I)>	19.8
Completeness [%]	97.7	81.4
Redundancy	3.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		Ammonium sulfate, HEPES, PEG 400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 100K
1	VAPOR DIFFUSION, SITTING DROP	7		Ammonium sulfate, HEPES, PEG 400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 100K
1	VAPOR DIFFUSION, SITTING DROP	7		Ammonium sulfate, HEPES, PEG 400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 100K