1RV5
COMPLEX OF ECORV ENDONUCLEASE WITH D(AAAGAT)/D(ATCTT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-15 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.600, 50.400, 63.900 |
| Unit cell angles | 96.40, 109.20, 108.50 |
Refinement procedure
| Resolution | 6.000 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.27200 |
| Starting model (for MR) | 1rva |
| RMSD bond length | 0.013 |
| RMSD bond angle | 23.622 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.078 |
| Total number of observations | 128913 * |
| Number of reflections | 29460 |
| Completeness [%] | 80.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 17 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | EcoRV | 10 (mg/ml) | |
| 2 | 1 | 1 | DNA | 1.0 (mg/ml) | |
| 3 | 1 | 1 | 5 (mM) | ||
| 4 | 1 | 1 | 0.3 (mM) | ||
| 5 | 1 | 1 | 30 (mM) | ||
| 6 | 1 | 2 | HEPES | 100 (mM) | pH7.5 |
| 7 | 1 | 2 | 150 (mM) | ||
| 8 | 1 | 2 | PEG4000 | 10-15 (%) |
