1RV4
E75L MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-02-06 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9363 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.040, 115.040, 158.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.510 - 2.950 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.24100 |
Structure solution method | Rigid body refinement of another E75L rcSHMT structure |
Starting model (for MR) | 1rv3 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.400 |
Data reduction software | DPS |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.500 | 3.110 |
High resolution limit [Å] | 2.950 | 2.950 |
Number of reflections | 23067 | |
<I/σ(I)> | 6.1 | 1.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9 | 10.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | PEG 8000, KCl, potassium phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |