1RV0

1930 Swine H1 Hemagglutinin complexed with LSTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-08-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	C 1 2 1
Unit cell lengths	202.040, 82.940, 176.880
Unit cell angles	90.00, 105.86, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.214
Rwork	0.214
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ruy
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.076	0.438
Number of reflections	85973
<I/σ(I)>	15.9	2
Completeness [%]	88.2	78.2
Redundancy	10.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291	PEG3350, TrisHCl, Sodium citrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K