1RUY

1930 Swine H1 Hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-01
Detector	ADSC QUANTUM 4
Spacegroup name	C 1 2 1
Unit cell lengths	203.106, 83.279, 178.234
Unit cell angles	90.00, 106.67, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.248
Rwork	0.248
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ru7
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.820
High resolution limit [Å]	2.700	2.700
Rmerge	0.084	0.324
Number of reflections	73352
<I/σ(I)>	7.8	2
Completeness [%]	90.5	68.4
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291	Peg5K MME, TrisHCl, Sodium citrate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K