1RUW
Crystal structure of the SH3 domain from S. cerevisiae Myo3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.330, 48.000, 78.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.17759 |
| Rwork | 0.173 |
| R-free | 0.24611 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.018 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.084 | 0.295 |
| Number of reflections | 7337 | |
| <I/σ(I)> | 12.7 | 3.8 |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Ammonium sulfate, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
