1RU7
1934 Human H1 Hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 227.515, 131.084, 174.383 |
| Unit cell angles | 90.00, 109.81, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jsd |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.097 | 0.478 |
| Number of reflections | 199084 | |
| <I/σ(I)> | 9.7 | 1.8 |
| Completeness [%] | 93.5 | 47.8 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 18% PEG3350, TrisHCl, KSCN, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
