1RTV
RmlC (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) crystal structure from Pseudomonas aeruginosa, apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-10 |
| Detector | MAC Science DIP-2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.833, 57.833, 161.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.230 - 2.500 |
| R-factor | 0.23326 |
| Rwork | 0.230 |
| R-free | 0.28971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dzr |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.902 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 2.670 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.094 | 0.455 |
| Number of reflections | 9710 | |
| <I/σ(I)> | 6.8 | 4 |
| Completeness [%] | 99.2 | 94.4 |
| Redundancy | 7.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Sodium tartrate, 0.1M MOPS, 8% PEG 8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
