1RTF

COMPLEX OF BENZAMIDINE WITH THE CATALYTIC DOMAIN OF HUMAN TWO CHAIN TISSUE PLASMINOGEN ACTIVATOR [(TC)-T-PA]

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	59.200, 59.200, 136.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.184
Rwork	0.184
R-free	0.26500
RMSD bond length	0.010
RMSD bond angle	1.640 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.300	2.300 *
Rmerge	0.066	0.333 *
Total number of observations	65287 *
Number of reflections	12894
Completeness [%]	99.8	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop		10 (mM)
3	1	drop	benzamidine	10 (mM)
4	1	reservoir	HEPES	0.2 (M)
5	1	reservoir	ethanol	20 (%(v/v))	can be replaced by isopropanol