1RT8

CRYSTAL STRUCTURE OF THE ACTIN-CROSSLINKING CORE OF SCHIZOSACCHAROMYCES POMBE FIMBRIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2003-03-12
Detector	SBC-3
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	84.243, 84.243, 150.819
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.000
Rwork	0.237
R-free	0.27000
Structure solution method	AB INITIO PHASING
Starting model (for MR)	1pxy
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	37818
Completeness [%]	95.2	65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	298	PEG 3300, MES, Lithium Sulfate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	5.7	298	PEG 3300, MES, Lithium Sulfate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K