1RT7

HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH UC84

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	137.600, 109.500, 72.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000
R-factor	0.252 *
Rwork	0.258
R-free	0.33400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	RT-MKC-442 COMPLEX (PDB ENTRY 1RT1)
RMSD bond length	0.009
RMSD bond angle	23.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.103	0.451
Total number of observations	53675 *
Number of reflections	19595	1453 *
<I/σ(I)>	8.6	1.73
Completeness [%]	87.3	70.8 *
Redundancy	2.74	1.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5	4 *	pH 5.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	citric acid	24.25 (mM)
2	1	1	sodium phosphate	51.5 (mM)
3	1	1	PEG3400	6-10 (%(w/v))