1RT7
HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH UC84
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.600, 109.500, 72.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| R-factor | 0.252 * |
| Rwork | 0.258 |
| R-free | 0.33400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RT-MKC-442 COMPLEX (PDB ENTRY 1RT1) |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.103 | 0.451 |
| Total number of observations | 53675 * | |
| Number of reflections | 19595 | 1453 * |
| <I/σ(I)> | 8.6 | 1.73 |
| Completeness [%] | 87.3 | 70.8 * |
| Redundancy | 2.74 | 1.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5 | 4 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | citric acid | 24.25 (mM) | |
| 2 | 1 | 1 | sodium phosphate | 51.5 (mM) | |
| 3 | 1 | 1 | PEG3400 | 6-10 (%(w/v)) |
