1RT2
CRYSTAL STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE COMPLEXED WITH TNK-651
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BL19 |
| Synchrotron site | ESRF |
| Beamline | BL19 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-22 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 137.300, 110.200, 72.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.550 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RT-MKC-442(1RT1) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.218 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.640 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.067 | 0.279 |
| Total number of observations | 233000 * | |
| Number of reflections | 30972 | 2508 * |
| <I/σ(I)> | 17.3 | 2.9 |
| Completeness [%] | 85.3 | 70.6 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | 4 * | pH 5.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3400 | 6 (%) | |
| 2 | 1 | drop | citric acid | 24.25 (mM) | |
| 3 | 1 | drop | 51.5 (mM) | ||
| 4 | 1 | reservoir | PEG3400 | 46 (%) |
